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About this book
About this book
Chemical biology is the use of chemical methods to investigate biological systems. This can include the synthesis of molecules similar to natural molecules, or creating small chemical changes to natural molecules. Another technique is to attach a chemical group to a natural molecule to either act as a marker, or to create chemical changes under specific conditions. Using these techniques scientists are probing the nature of biochemical molecules and systems.
This text focuses on the methods for obtaining modified and native nucleic acids, and their biological applications.
Foreword. Preface. List of Contributors. 1 Chemical Synthesis of Modified RNA (Claudia Hobartner and Falk Wachowius). 2 Expansion of the Genetic Alphabet in Nucleic Acids by Creating New Base Pairs (Ichiro Hirao and Michiko Kimoto). 3 Chemical Biology of DNA Replication: Probing DNA Polymerase Selectivity Mechanisms with Modified Nucleotides (Andreas Marx). 4 Nucleic Acid-templated Chemistry (Michael Oberhuber). 5 Chemical Biology of Peptide Nucleic Acids (PNAs) (Peter E. Nielsen). 6 The Interactions of Small Molecules with DNA and RNA (Yun Xie, Victor K. Tam and Yitzhak Tor). 7 The Architectural Motifs of Folded RNAs (Valerie Fritsch and Eric Westhof). 8 Genesis and Biological Applications of Locked Nucleic Acids (LNAs) (Harleen Kaur and Souvik Maiti). 9 Small Non-coding RNA in Bacteria (Sabine Brantl). 10 MicroRNA-guided Gene Silencing (Gunter Meister). 11 Nucleic Acid-based Therapies (Britta Hoehn and John J. Rossi). 12 Innate Immune Recognition of Nucleic Acids (Stefan Bauer). 13 Light-responsive Nucleic Acids for the Spatiotemporal Control of Biological Processes (Alexander Heckel and Gunter Mayer). 14 DNA Methylation (Albert Jeltsch and Renata Z. Jurkowska). 15 Frameworks for Programming RNA Devices (Maung Nyan Win, Joe C. Liang and Christina D. Smolke). 16 RNA as a Catalyst: The Diels-Alderase Ribozyme (Andres Jaschke). 17 Evolving an Understanding of RNA Function by In Vitro Approaches (Qing Wang and Peter J. Unrau). 18 The Chemical Biology of Aptamers: Synthesis and Applications (Gunter Mayer and Bernhard Wulffen). 19 Nucleic Acids as Detection Tools (Jeffrey C.F. Lam, Sergio Aguirre and Yingfu Li). 20 Bacterial Riboswitch Discovery and Analysis (Tyler D. Ames and Ronald R. Breaker). Index.