Gene Probes: A Practical Approach, Volume 2 Applications

About this book

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to the analysis of human genetic mutations. A companion volume covers the preparation of these probes. The volumes aim to contribute to a growing field of research.

Contents

1. Hybridization strategy; 2. Screening recombinant DNA libraries by hybridization and amplification; 3. Screening human recombinant libraries with nucleic acid probes; 4. Antibody screening of bacterial expression systems; 5. Isolation of cDNA clones by direct expression in mammalian cells; 6. Genomic mapping and gene structure analysis; 7. Analysis of gene expression by blotting techniques; 8. In situ hybridization of nucleic acid probes to cellular RNA; 9. Applications of chromosomal in situ hybridization; 10. Analysis of gene expression analysis by PCR: a quantitative approach; 11. Analysis of protein expression by immunocytochemistry; 12. Gene targeting in mammalian cells; 13. Directed mutagenesis and mutant analysis; 14. Human gene mutational analysis; Appendix 1; Appendix 2

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