Plant Chromosomes Laboratory Methods

About this book

The only single-volume resource that covers all important approaches to the study of plant chromosomes. |It reviews each specific approach and describes experimental techniques, and provides the best methods for improving plant chromosome research.

Contents

Plant Chromosomes at Mitosis, K. Fukui Outline of Mitotic Chromosomes Preparation Method for the Small Chromosome: Enzymatic Maceration/Air Drying (EMA) Method Preparation Method for the Large Chromosome: Squash Method Preparation Method for Chromosomal Samples of Woody Plants Preparation Method for Chromosome Samples of Suspension Cells Preparation Method for Chromosome Samples of Flowers and Leaves Permanent Preparation of Chromosome Samples Plant Chromosomes at Meiosis, P.G. Goicoechea and R. Giraldez Introduction Meiotic Synchrony and Meiotic Gradients Procedures Plant Chromosomes at Metabolic Phase, K. Taniguchi Introduction Morphological Classification by Distribution of Condensed Heterochromatin Visualizing Condensation Application of Chromosome Banding Techniques Polytene Chromosomes, W. Nagl Introduction Occurrence and Structure of Polytene Chromosomes in Plants Preparation Staining Methods DNA and Gene Analysis Digitation and Computer Analysis Future Challenges Flow Cytometry and Chromosome Sorting, J.S. Heslop-Harrison and T. Schwarzacher Introduction Procedures Future Applications Chromosome Dissection and Direct Cloning, Y. Kamisugi and K. Fukui Introduction Equipment Microdissection of Plant Chromosomes Direct Cloning Direct-Labeling and In Situ Hybridization Chromosome Banding Methods, B. Friebe, T.R. Endo, and B.S. Gill Introduction Principles of Chromosome Banding Procedures In Situ Hybridization, Y. Mukai Introduction Advances in In Situ Hybridization Procedures Sister Chromatid Exchange and Replication Banding, B. Friebe and F. Cortes Introduction Sister Chromatid Differentiation in Second Division Chromosomes Sister Chromatid Differentiation in Third Division Chromosomes Replication Banding Procedures Replication of Chromosomes, K. Taniguchi and I. Fujishige Introduction Principles of Replication Banding Procedures Applications Chromosome Manipulation in Wheat, J.P. Gustafson and J.W. Snape Introduction Genome Balance and Introgression Random Homologous Alien Transfers Irradiation Technologies and Selection Strategies Homologous Transfers by the Control of Recombination Alien Introgression and Gene Expression Conclusions Electron Microscopy and Plant Chromosomes, J.S. Heslop-Harrison Introduction Developments in Electron Microscopy General Precautions for Electron Microscopy The Specimen Procedure DNA:DNA In Situ Hybridization Quantification and Reconstruction of Three Dimensional Images from Serial Sections Transfer of Spread Chromosome Preparations from Slides to Electron Microscope Developments and Conclusions Analysis of Chromosome Information, K. Fukui and S. Nakayama Historical Perspective of Imaging Plant Chromosomes Concept of Image Analyses Instrumentation and Software Procedure Appendix I: Buffer Solutions Appendix II: Tissue Culture Media Index

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