750 pages, Illus, figs, tabs
This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications. It features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies. "Recombinant DNA Principles and Methodologies" discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as plasmid biology, nucleic acid biochemistry, restriction enzymes, cloning strategies, gel electrophoresis, southern and northern blotting, preparation of probes, phage lambda biology, cosmids and genome analysis, cloned gene expression, polymerase chain reaction ,conventional and automated DNA sequencing site-directed mutagenesis and more! Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits. - Trends in Genetics
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