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The use of animal, including human, cell culture expanded enormously during the last 25 years of the 20th century, with new applications appearing all the time. The aim of this book is to guide the newcomer progressively through all those areas which nowadays are basic to the performance of cell culture. The book should also prove useful to the experienced worker when entering a new field, setting up a new laboratory, or as a source of reference on basic techniques. Revised and updated chapters cover topics such as equipping a cell culture laboratory, sterilization, culture media, basic culture technique, cell cloning, the isolation of new cell lines, contamination procedures, and good laboratory practice.
"If you are planning to set up any functional cell structure facility, small- or large-scale, Basic Cell Structure could be very useful. The book has high practical and scientific value. . .The text is compact and it is easy to find specific information. . .Overall, the book provides a guide for any university of pharmaceutical company laboratory, with or without good laboratory practise (GLP) standards, that aims to obtain consistent cell biological data. I suggest this book cold serve as a handy reference guide and recommend it for managers of cell structure facilities and any person practising cell structure work. . .[T]his book takes on a wider and more conclusive attitude to the cell structure process. Personally, I have placed it in the lab where I am working in, and my colleagues and I have found it to be very handy when working at the bench or planning future studies."--Journal of Cell Science
"The second edition contains a new chapter--on microscopy--updated information
Caroline Wigley, The Cell Culture Laboratory; Peter Roberts, Sterilization; T. Cartwright and G.P. Shah, Culture Media; Ian Dobbie and Daniel Zicha, Microscopy of Living Cells; James McAteer and John M. Davis, Basic cell culture and the maintenance of cell lines; Caroline McDonald, Primary culture and the establishment of cell lines; J.D. Sato et al, Specific cell types and their requirements; John Clarke, Alison Porter, Robin Thorpe, and John M Davis, Cloning; B.J. Bolton, P. Packer and A. Doyle, The quality control of cell lines; Barbara Clitherow, Good laboratory practice in the cell culture laboratory. Appendix: J. Dow, Online resources for cell biology.
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