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Covers a range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green macromolecular location procedures involving immunocytochemistry and in situ hybridisation.
INTRODUCTION TO OPTICAL MICROSCOPY FOR PLANT CELL BIOLOGY; FLUORESCENT PROBES FOR LIVING PLANT CELLS; FLOW CYTOMETRY; TRANSIENT EXPRESSION, A TOOL TO ADDRESS QUESTIONS IN PLANT CELL BIOLOGY; THE GREEN FLUORESCENT PROTEIN (GFP) AS REPORTER IN PLANT CELLS; MICROINJECTION; MICROMANIPULATION BY LASER MICROBEAM AND OPTICAL TWEEZERS; ELECTROPHYSIOLOGICAL METHODS: MONITORING EXO- AND ENDOCYTOSIS IN REAL TIME; PLANT HISTOLOGY; IMMUNOCYTOCHEMISTRY FOR LIGHT MICROSCOPY; ELECTRON MICROSCOPY; IN SITU HYBRIDIZATION; ORGANELLE ISOLATION
... an excellent guide for advanced students, postgraduates and researchers to handle techniques in plant cell biology. Journal of Plant Physiology The layout of the book is clear, with highly informative illustrations ... For anyone curious about cell biology, this book provides an excellent introduction to the major relevant techniques and works well as a laboratory manual. Trends in Plant Science, 2002