All Shops
We're still open for business, read our Covid-19 statement here

British Wildlife

6 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published six times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £30 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £18 per year
Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Crystallization of Nucleic Acids and Proteins: A Practical Approach

Handbook / Manual
Edited By: A Ducruix and R Giege
435 pages, B/w plates, figs, tabs
Crystallization of Nucleic Acids and Proteins: A Practical Approach
Click to have a closer look
  • Crystallization of Nucleic Acids and Proteins: A Practical Approach ISBN: 9780199636785 Edition: 2 Paperback Oct 1999 Usually dispatched within 2-3 weeks
    £107.50
    #120806
Price: £107.50
About this book Contents Customer reviews Related titles

About this book

Crystallography is the major method of determining structures of biological macromolecules yet crystallization techniques are still regarded as difficult to perform. This new edition of Crystallization of Nucliec Acids and Proteins: A Practical Approach continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two- dimensional electron crystallography of soluble proteins on planar lipid films. As well as an introduction to crystallogenesis, the other topics covered are: Handling macromolecular solutions, experimental design, seeding, proceeding from solutions to crystals Crystallization in gels Crystallization of nucleic acid complexes and membrane proteins Soaking techniques Preliminary characterization of crystals in order to tell whether they are suitable for diffraction studies. As with all Practical Approach books the protocols have been written by experienced researchers and are tried an tested methods. The underlying theory is brought together with the laboratory protocols to provide researchers with the conceptual and methodological tools necessary to exploit these powerful techniques. Crystallization of Nucliec Acids and Proteins: A Practical Approach 2e will be an invaluable manual of practical crystallization methods to researchers in molecular biology, crystallography, protein engineering, and biological chemistry.

Contents

Preface; An introduction to the crystallogenesis of biological macromolecules; Biochemical aspects and handling of macromolecular solutions and crystals; Molecular biology for structural biology; Experimental design, quantitive analysis, and the cartography of crystal growth; Methods of crystallization; Crystallization in gels and related methods; Seeding techniques; Nucleic acids and their complexes; Crystallization of membrane proteins; From solution to crystals with a physico-chemical aspect; Diagnostic of prenucleation and nucleation by spectroscopic methods and background on the physics; Two-dimensional crystallization of soluble proteins on planar lipid films; Soaking techniques; X-ray analysis; Index

Customer Reviews

Handbook / Manual
Edited By: A Ducruix and R Giege
435 pages, B/w plates, figs, tabs
Current promotions
Handbook of the mammals of the world batsJohn Beaufoy PublishingNest Box Price List 2020Order your free copy of our 2020 equipment catalogue