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A Practical Guide to Protein and Peptide Purification for Microsequencing

Handbook / Manual

By: Paul Matsudaira(Editor)

184 pages, illustrations

Academic Press

Spiralbound | Jul 1993 | Edition: 2 | #34713 | ISBN: 0124802826
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £43.99 $57/€50 approx

About this book

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. The highlights of the Second Edition include: mass spectrophotometric characterization of proteins and peptides; N-terminal sequencing of proteins with blocked N termini; Internal amino acid sequence analysis after protease digestion in-gel and on-blot; improved microscale peptide purification methods; computer analysis of protein sequences; new protocols tested and refined through everyday use in authors' laboratories; and, updated reference chapter covering all aspects of protein microsequencing.

From the Reviews of the First Edition:

"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world."


- Introduction
- Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein
- Enzymatic Digestion of Proteins and HPLC Peptide Isolation
- Purification of Proteins and Peptides By SDS-PAGE
- Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose
- Mass Spectrometric Strategies for the Structural Characterization of Proteins
- Appendix
- References
- General Reference Books on Protein Microsequencing Methodologies

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