To see accurate pricing, please choose your delivery country.
 
 
United States
£ GBP
All Shops

British Wildlife

8 issues per year 84 pages per issue Subscription only

British Wildlife is the leading natural history magazine in the UK, providing essential reading for both enthusiast and professional naturalists and wildlife conservationists. Published eight times a year, British Wildlife bridges the gap between popular writing and scientific literature through a combination of long-form articles, regular columns and reports, book reviews and letters.

Subscriptions from £33 per year

Conservation Land Management

4 issues per year 44 pages per issue Subscription only

Conservation Land Management (CLM) is a quarterly magazine that is widely regarded as essential reading for all who are involved in land management for nature conservation, across the British Isles. CLM includes long-form articles, events listings, publication reviews, new product information and updates, reports of conferences and letters.

Subscriptions from £26 per year
Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

A Practical Guide to the Study of Calcium in Living Cells

Handbook / Manual
Series: Methods in Cell Biology Volume: 40
Edited By: R Nuccitelli
368 pages, 7 col plates, illus, figs, tabs
Publisher: Academic Press
A Practical Guide to the Study of Calcium in Living Cells
Click to have a closer look
Select version
  • A Practical Guide to the Study of Calcium in Living Cells ISBN: 9780125228107 Spiralbound May 1994 Not in stock: Usually dispatched within 1-2 weeks
    £43.99
    #34801
  • A Practical Guide to the Study of Calcium in Living Cells ISBN: 9780125641418 Hardback Apr 1994 Not in stock: Usually dispatched within 1-2 weeks
    £43.99
    #35212
Selected version: £43.99
About this book Contents Customer reviews Related titles

About this book

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. This title describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes, fluorescent dyes, and luminescent proteins. It includes techniques for perturbing intracellular Ca2+. It covers detailed methodology plus problems and pitfalls of each technique. It contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program. The color plates illustrate techniques such as confocal ratio-imaging and use of aequorin.

Contents

Ca2+ Buffers and [Ca2+]i Perturbation Techniques: D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers. R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+. D. Thomas and M. Hanley, Pharmacological Tools for Perturbing Intracellular Ca2+ Stores. Microelectrode Techniques for Measuring [Ca2+]i and Ca2+ Fluxes: S. Baudet, L.H. Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes. P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux. C. Leech and G.G. Holz, IV, Application of PatchClamp Methods to the Study of Calcium Currents and Calcium Channels Fluorescence Techniques for Imaging [Ca2+]i: J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators. S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH. T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques. P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells. S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes. K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]i Using a Photomultiplier Tube. A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]i with Aequorin Using a Photon Imaging Detector. R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles. Subject Index. References.

Customer Reviews

Handbook / Manual
Series: Methods in Cell Biology Volume: 40
Edited By: R Nuccitelli
368 pages, 7 col plates, illus, figs, tabs
Publisher: Academic Press
Media reviews

This multiauthored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced...The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research. --RAMESH BHALLA, University of Iowa, Iowa City "The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research." --MOLECULAR & CELLULAR NEUROSCIENCES

Current promotions
New and Forthcoming BooksNHBS Moth TrapBritish Wildlife MagazineBuyers Guides