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A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. This title describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes, fluorescent dyes, and luminescent proteins. It includes techniques for perturbing intracellular Ca2+. It covers detailed methodology plus problems and pitfalls of each technique. It contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program. The color plates illustrate techniques such as confocal ratio-imaging and use of aequorin.
Ca2+ Buffers and [Ca2+]i Perturbation Techniques: D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers. R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+. D. Thomas and M. Hanley, Pharmacological Tools for Perturbing Intracellular Ca2+ Stores. Microelectrode Techniques for Measuring [Ca2+]i and Ca2+ Fluxes: S. Baudet, L.H. Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes. P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux. C. Leech and G.G. Holz, IV, Application of PatchClamp Methods to the Study of Calcium Currents and Calcium Channels Fluorescence Techniques for Imaging [Ca2+]i: J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators. S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH. T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques. P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells. S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes. K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]i Using a Photomultiplier Tube. A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]i with Aequorin Using a Photon Imaging Detector. R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles. Subject Index. References.
This multiauthored book... provides theoretical and applied aspects of different established techniques for the measurement of [Ca2+]i at submicromolar concentration. Each chapter is organized, keeping in view the needs of a person working in the laboratory, by giving the detailed methodology required for the implementation of that particular technique, and all the chapters are well referenced...The book provides a pragmatic approach for the estimation of cytosolic [Ca2+]i and also provides a means for studying the spatial and temporal resolution of [Ca2+]i. The book achieves the stated goal in the preface, 'this material will be very useful to the investigator who wants to apply any of these methods to his or her research. --RAMESH BHALLA, University of Iowa, Iowa City "The book achieves the stated goal in the preface, this material will be very useful to the investigator who wants to apply any of these methods to his or her research." --MOLECULAR & CELLULAR NEUROSCIENCES