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Advances in Genome Biology, Volume 1: Unfolding the Genome

Out of Print

Edited By: RS Verma

JAI Press

Hardback | Dec 1991 | #31329 | ISBN: 1559383496
Out of Print Details

About this book

This volume includes twelve chapters. The first chapter offers an overview on gene mapping and sequencing. The remaining eleven chapters are devoted to the methods most routinely employed in both clinical and basic research. A brief theoretical basis of each procedure has also been included. A request was made to each contributor to describe the protocols within the field of their expertise, as the intent of this issue is to promote awareness of current technical progress. Readers of this volume will find these experimental procedures highly applicable to their research endeavours.


Contents. List of Contributors. Introduction to the Series (R.S. Verma). Genetic Techniques for Mapping and Sequencing the Genome: An Overview (R.S. Verma). Cloning Defined Regions of the Human Genome by Microdissection of Banded Chromosomes and Enzymatic DNA Amplification (U. Claussen, G. Senger, H. Ludecke, and B. Horsthemke). In SITU Hybridization with Radiolabeled Probes (P. Szabo). Analysis of Genome DNA by Southern Blotting (P. ten Dijke and K. Stam). Analysis of RNA by Northern Hybridization (A. Kumar and M.A.Q. Siddiqui). Slot-Blot Technique: Principles and Applications (G. Vasanthakumar). Spot-Blot Gene Mapping and Bivariate Flow Cytogenetics (R.V. Lebo). Polymerase Chain Reaction: Principles and Applications (S.M. Carleton). Protocols for Pulsed Field Gel Electrophoresis: Separation and Detection of Large DNA Molecules (R.M. Gemmill, J. Coyle-Morris, P. Scott, S. Paulien, M. Mendez, and M. Varella-Garcia). Detection of Single Base Changes in Nucleic Acid (R.G.H. Cotton). Identification of Chromosome-Specific Satellite DNA Using Non-Isotopic in KITU Hybridization (M. Adinolfi). Construction and Use of Lambda and Cosmid Linkage Libraries (A.R. Kimmel). Index.

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