About this book
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.
Principles of cell culture: Animal cell culture equipment and techniques, D. Barnes; Making informed choices - medium, serum, and serum-free medium - how to choose the appropriate medium and culture system for the model you wish to create, J.P. Mather; Cell line availability - where to get the cell lines you need, R.J. Hay; Cell culture contamination - sources, consequences, prevention, and elimination, M.G. Gabridge. Establishing cell lines: Immortalization by gene transfection, Y. Katakura et al; Establishment of mammalian testicular cell lines, M.-C. Hoffmann, J.L. Millan; Cell hybridization, hybridomas, and human hybridomas, S. Shirahata et al; establishing human glioma-derived cell lines, M. Westphal, H. Meissner; Culture methods for selective growth of normal rat and human Schwann cells, R. Li; Invertebrate cell culture considerations - insects, ticks, shellfish, and worms, C.J. Bayne; Cell line characterization and authentication, J. Kaplan, B. Hukku. Specialized culture techniques: Laboratory scale-up of cell cultures (0,5-50 litres), J.P. Mather; Cell synchronization, G.F. Merrill; Measurement of cell death, D.T. Loo, J.R. Tillema; Simultaneous measurement of cell cycle and apoptotic cell death, A. Moore et al; Embryonic stem cells, creating transgenic animals, M. Pirity et al. Microscopy and morphology: Electron microscopy - use of transmission and scanning electron microscopy to study cells in culture, D.M. Phillips; Indirect immuno-fluorescence microscopy in cultured cells, S.P. Wheatly, Y.-L.Wang; Cellular localization of mRNA and protein - in situ hybridization histochemistry and in situ ligand binding, T.K. Woodruff.
Jennie P. Mather, Residence: San Francisco, California. Affiliation: Genentech, Inc., San Francisco, California. - David Barnes, Residence: Corvallis, Oregon. Affiliation: Oregon State University, Corvallis, Oregon.