Application of Sampling and Detection Methods in Agricultural Plant Biotechnology describes detection methods for seed, plants and grain derived from biotechnology. This international handbook, based on a series of workshops carried out for governments in collaboration with ILSI and Co-published in partnership with the Cereals & Grains Association, provides the technical and practical information needed to develop, validate and use detection methods. This useful resource provides readers with the tools necessary to carry out reliable sampling, detection and interpretation of data.
1. Introduction: Biotechnology and Genetically Modified Crops
2. Seed Testing – Purity and Low-Level Presence (LLP)
3. Grain Supply Chain Testing – Import and Export
4. Principles of Nucleic Acid-Based Detection Methods
5. Method Validation: DNA Based Detection Methods
6. Principles of Protein Detection Methods
7. Method Validation: Protein Detection Methods
8. Sources and Use of Reference Materials
9. Seed and Grain Sampling
10. Plant and Field Analysis
11. Testing Laboratory Design and Management
12. International Standards
13. Analytical Strategy and Interpretation of Results
14. Detection of Gene-Edited Products
15. Future Perspectives and Challenges
Ray Shillito was a co-organizer of the ILSI International Food Biotechnology Committee (IFBiC)– American Association of Cereal Chemists International (AACCI) workshops from which the concept and much of the content of this book were derived and has presented the materials in the workshops given in many countries. As a BASF business support manager, he represents BASF (Seeds and Traits) in many trade associations and technical and science organizations, including CropLife, ISO, AOAC, Cereals & Grains Association (formerly AACC), and AEIC. He interacts internationally on standards setting in the area of analytical technology (with a focus on agricultural biotechnology), and he chairs the ISO committee TC34/SC16 (Biomarkers) and the Cereals & Grains Association Molecular Biomarkers for Grain Technical Committee.
Guomin Shan received a bachelor’s degree in pesticide chemistry and a master’s degree in environmental toxicology from Beijing Agricultural University, China. In 1997, he received a PhD degree in entomology from Louisiana State University. After graduating, Guomin spent 4 years at the University of California, Davis as a postdoctoral fellow and a research scientist. He joined Dow AgroSciences LLC in 2001, where the focus of his career has been on transgenic traits and animal health product research and development. At Dow, he has served in various leadership positions, including technical leader, science leader, seeds regulatory business partner, and global regulatory leader. Currently, he is the leader of the Predictive Safety Center in Corteva Crop Protection Regulatory Science.