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&i;Bioinformatics: A Practical Approach&o; presents quick and efficient applications through step-by-step instructions and examples. With an overview in each chapter, this book demonstrates how to screen out genes of interest from genomics, transcriptomics, proteomics, and other integrative databases and shows how to retrieve single nucleotide polymorphism and haploytype data. The book also describes various programs and tools in DNA and protein sequence analysis and introduces basic biocomputing tools. Mice database mining is also covered. Along with sample datasets and a list of useful websites and databases, this resource features contributions from leading international experts in the field.
GENOME AND DNA SEQUENCE ANALYSISGenome AnalysisGenome structure analysis by genome browserSequence similarity searching by BLASTTwo Common DNA Analysis ToolsRestriction mappingPCR applicationPhylogenetics AnalysisMultiple sequence alignmentsBuilding phylogenetics treeSNP and Haplotype AnalysisSNP analysisHaplotype analysisTRANSCRIPTOME AND RNA SEQUENCE ANALYSISGene Expression Profiling by MicroarrayExperimental designData acquisition and preprocessingData analysisTutorialGene Expression Profiling by SAGESAGE experimentationSAGE data analysisRetrieve data from SAGE databaseRegulation of Gene ExpressionAlternative promotersAlternatively splicingAlternative translation initiationRNA editingMicroRNoma Genome-Wide Profiling by MicroarrayWhat are miRNAs?microRNAs and human diseasesSignificance of microRNA profilingSignificance of microRNA CGH assayGenome-wide microRNA profiling by microarrayRNAiIntroduction of RNAi and siRNASearch for siRNA informationPROTEOME AND PROTEIN SEQUENCE ANALYSISProteome AnalysisAnalyzing 2DE-derived dataAnalyzing mass spectrometry derived dataAnalyzing data derived from protein microarraysProtein Sequence AnalysisPrimary structure analysisSecondary and tertiary structure analysisPattern and profile searchProtein Function AnalysisProtein function annotationProtein posttranslational modificationsProtein-protein interactionsFunctional Annotation of Proteins in Murine ModelsVarious murine models of human diseasesSearch for murine databasesBASIC BIOCOMPUTING TOOLSApplication of Programming Languages in BiologyApplication of Perl in biologyApplication of Java in biologyApplication of R in biologyWebsite and Database DesignPersonal website designNuts and bolts of biological databases and their constructionsMicrosoft Excel and AccessMicrosoft ExcelMicrosoft AccessInterchange data between Microsoft Excel and AccessAPPENDICESAbbreviationsSelected glossaryWebsites for selected bioinformatics softwaresINDEX
University of Missouri, Columbia, USA