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Academic & Professional Books  Organismal to Molecular Biology  Biochemistry & Molecular Biology

Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology

By: Bernhard Rupp
800 pages, col illus, tabs
Publisher: Garland Science
Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology
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  • Biomolecular Crystallography: Principles, Practice, and Application to Structural Biology ISBN: 9780815340812 Hardback Oct 2009 Usually dispatched within 6 days
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About this book

Synthesizing over thirty years of advances into a comprehensive textbook, this title describes the fundamentals, practices and applications of protein crystallography. Illustrated in full-color by the author, the text describes mathematical and physical concepts in accessible and accurate language. It distills key concepts for understanding the practice and analysis of protein crystal structures and contains examples of biologically-relevant molecules, complexes and drug target structures.

Contents

TABLE OF CONTENTS Preface PART I: FROM SEQUENCE TO CRYSTALS 1. Introduction: Preparing Your Study 2. Protein Structure 3. Protein Crystallization 4. Proteins for Crystallography PART II: FUNDAMENTALS OF PROTEIN CRYSTALLOGRAPHY 5. Crystal Geometry 6. Diffraction Basics 7. Statistics and Probability in Crystallography PART III: FROM CRYSTALS TO DATA 8. Instrumentation and Data Collection PART IV: DETERMINING YOUR STRUCTURE 9. Reconstruction of Electron Density and the Phase Problem 10. Experimental Phasing 11. Non-Crystallographic Symmetry and Molecular Replacement 12. Model Building and Refinement PART V: MAKING SENSE OF YOUR STRUCTURE 13. Structure Validation, Analysis, and Presentation Appendix Annotation Acknowledgements Tables Glossary Index

Customer Reviews

Biography

q.e.d. life science discoveries, inc. 6487 Half Dome Court Livermore, CA 94551
By: Bernhard Rupp
800 pages, col illus, tabs
Publisher: Garland Science
Media reviews
PRAISE FOR Biomolecular Crystallography: "This is the book that molecular biologists and the crystallographic community have been waiting for." Alexander McPherson, University of California at Irvine, USA a "Biomolecular Crystallography is first and foremost a comprehensive reference text and laboratory manual for the practicing structural biologist, from the basics of biomolecular structure to modern advanced and powerful techniques in biomolecular structure determination, and analysis and application of structural information.a Figures and graphs computed from new and original data are used extensively to help clarify important concepts, and derivations of all relevant mathematical and statistical principles--many of which have never been brought together in a single volume--are presented.a This fulfils a long-felt need!" Ian J. Tickle, Astex Therapeutics, Cambridge, UK "This thorough treatment of modern macromolecular X-ray crystallography combines a comprehensive coverage of the fundamentals with methodological details that are often omitted in introductory texts.a It will be of value to anyone that works in macromolecular X-ray crystallography, particularly to graduate students or postdoctoral fellows who are mastering the technique." Mark Wilson, University of Nebraska, USA "Given the extraordinary progress in biomolecular crystallography, the challenge of providing a comprehensive and authoritative overview, starting from first principles, is formidable. Dr, Rupp has, however, succeeded admirably." Brian Matthews, University of Oregon, USA
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