In Vitro Culture of Higher Plants

About this book

Presents an up-to-date and wide-ranging account of the techniques and applications, and has primarily been written in response to practical problems.

Contents

1. Preface. 2. Introduction. 3. History. 4. Types of Cultures. 5. Laboratory Equipment. 6. Preparation and Composition of Nutrient Media. 7. Closure of Test Tubes and Flasks. 8. Care of Plant Material. 9. Sterilization of Plant Material. 10. Isolation, Inoculation and Subculturing. 11. Mechanization. 12. The Influence of Plant Material on Growth and Development. 13. The Influence of Physcial Factors on Growth and Development. 14. The Transfer from Nutrient Medium to Soil. 15. Aids to Study. 16. Embryo Culture. 17. Germination of Orchid Seeds. 18. Vegetative Propagation of Orchids. 19. Production of Disease-Free Plants. 20. Vegetative Propagation. 21. Somaclonal Variation. 22. Test Tube Fertilization. 23. Production of Haploids. 24. Genetic Manipulation. 25. Miscellaneous Applications. 26. In vitro Cloning of Plants in the Netherlands.

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