Diagnostics in Plant Breeding is systematically organizing cutting-edge research reviews on the development and application of molecular tools for the prediction of plant performance. Given its significance for mankind and the available research resources, medical sciences are leading the area of molecular diagnostics, where DNA-based risk assessments for various diseases and biomarkers to determine their onset become increasingly available. So far, most research in plant genomics has been directed towards understanding the molecular basis of biological processes or phenotypic traits.
From a plant breeding perspective, however, the main interest is in predicting optimal genotypes based on molecular information for more time- and cost-efficient breeding schemes. It is anticipated that progress in plant genomics and in particular sequence technology made recently will shift the focus from "explanatory" to "predictive" in crop science. Diagnostics in Plant Breeding assembles chapters on all areas relevant to development and application of predictive molecular tools in plant breeding by leading authorties in their respective areas.
Section 1. Introduction
Chapter 1. Diagnostics in plants; T. Lubberstedt
Chapter 2. Non-DNA biomarkers; Christin Falke
Section 2. Identification of quantitative trait polymorphisms (QTPs)
Chapter 3. Gene identification: forward genetics; Qing Ji
Chapter 4. Gene identification: reverse genetics; George Haughn
Chapter 5. Allele re-sequencing technologies; Torben Asp
Chapter 6. Association studies; Yongsheng Chen
Section 3. Validation of QTPs
Chapter 7. TILLING; Gunter Backes
Chapter 8. Gene Replacement; Paul Hooykaas, Sylvia de Pater
Section 4. Conversion of QTPs into Functional Markers
Chapter 9. SNP genotyping technologies; Bruno Studer
Chapter 10. INDEL genotyping technologies; Toshihiko Yamada et al
Chapter 11. Sequencing for genotyping; Rajeev Varshney
Section 5. Development of Non-DNA Biomarkers
Chapter 12. Methylation-based markers; Emidio Albertini, Gianpiero Marconi
Chapter 13. RNA; Stefan Scholten
Chapter 14. Metabolites; Olga Zabotina
Section 6. Deposition of Diagnostic Marker information
Chapter 15. Plant Genetic Databases; Carolyn Lawrence
Section 7. Statistical considerations
Chapter 16. Epistasis, QTP x environment interactions, and pleiotropy: value of a FM; Everton Brenner
Section 8. Applications in plant breeding
Chapter 17. Parent selection - usefulness; Adel Abdel-Ghani
Chapter 18. Variety registration and protection; David Lee et al
Section 9. Examples
Chapter 19. Maize; Mingliang Xu et al
Chapter 20. Rice; Yongzhong Xing et al
Chapter 21. Traits; Birgit Arnholdt-Schmitt, Helia Cardoso