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DNA Cloning: A Practical Approach, Volume 4: Mammalian Systems
Handbook / Manual
Series: DNA Cloning: A Practical Approach Volume: 4
Edited By: BD Hames and D Glover
302 pages, 13 b/w photos, 26 line illus
About this book
Topics include: production of transgenic animals; adnenovirus vectors; retroviral vectors.
Contents
1. High efficiency gene transfer into mammalian cells; 2. Construction and characterization of Vaccinia virus recombinants; 3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells; 4. Retroviral vectors; 5. Genetic manipulation of embryonic stem cells; 6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs; 7. Genomic and expression analysis of transgenic animals; 8. Expression using a defective Herpes simplex virus (HSV-1) vector system; 9. Adenovirus vectors
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