DNA Cloning: A Practical Approach, Volume 4: Mammalian Systems

Topics include: production of transgenic animals; adnenovirus vectors; retroviral vectors.

1. High efficiency gene transfer into mammalian cells; 2. Construction and characterization of Vaccinia virus recombinants; 3. Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells; 4. Retroviral vectors; 5. Genetic manipulation of embryonic stem cells; 6. Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs; 7. Genomic and expression analysis of transgenic animals; 8. Expression using a defective Herpes simplex virus (HSV-1) vector system; 9. Adenovirus vectors