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This laboratory guide for successful electrophoretic separations is divided into two parts to provide thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
Part I Fundamentals: electrophoresis; isotachophoresis; isoelectric focusing; blotting; interpretation of electropherograms; proteome analysis; instrumentation; equipment for part II. Part II Methods: method 1 - PAGE of dyes; method 2 - agarose and immuno electrophoresis; method 3 - titration curve analysis; method 4 - native PAGE in amphoteric buffers; method 5 - agarose IEF; method 6 - PAGIEF in rehydrated gels; method 7 - horizontal SDS-PAGE; method 8 - vertical PAGE; method 9 - semi-dry blotting of proteins; method 10 - IEF in immobilized pH gradients; method 11 - high-resolution 2D electrophoresis; method 12 - PAGE of double stranded DANN; method 13 - native PAGE of single stranded DNA7; method 14 - denaturing gradient gel electrophoresis; method 15 - denaturing PAGE of DANN. Appendix: Trouble-shooting guide.
Born in 1951, Reiner Westermeier gained his diploma in engineering in 1976, and his doctorate in 1981 at the Technical University of Munich, Germany. He remained at the TU engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours. From 1984 to 1987 Dr. Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech since July 1997, and its Senior Scientist for Proteomics since January 2002. Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.
...an excellent reference to help the reader to become acquainted with the field of electrophoresis. (Clinical Chemistry) "...a practical guide..." (SciTech Book News, Vol. 26, No. 2, June 2002)