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Contents
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About this book
Manual that covers all up-to-date procedures necessary for purifying, characterizing and handling of proteins and enzymes.
Contents
Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Personal Perspectives on Enzyme Purification. Index.
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