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Summarizes the key principles underlying enzyme assays, including assay design and the evaluation of experimental data.
1. Principles of enzyme assay and kinetic studies; 2. Photometric assays; 3. Radiometric assays; 4. High performance liquid chromatography assays; 5. Electrochemical assays: the oxygen electrode; 6. Electrochemical assays: the nitric oxide electrode; 7. Electrochemical assays: the pH-stat; 8. Enzyme assays after gel electrophoresis; 9. Techniques for enzyme extraction; 10. Determination of active site concentration; 11. High throughput screening - considerations for enzyme assays; 12. Statistical analysis of enzyme kinetic data
Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes. Natural Product Reports