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Enzyme Assays: Essential Data

Series: Essential Data

Edited By: S Gul, SK Sreedharan and K Brocklehurst

118 pages, Tabs

Oxford University Press

Paperback | Apr 2002 | Edition: 2 | #126148 | ISBN: 0199638209
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £84.99 $112/€95 approx
Hardback | Apr 2002 | Edition: 2 | #126149 | ISBN: 0199638217
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £145.00 $190/€162 approx

About this book

Summarizes the key principles underlying enzyme assays, including assay design and the evaluation of experimental data.

Provides a very detailed and useful discussion of practical issues in kinetic analysis, illustrates with relevant examples, and provides primary references to the biochemical literature, which are up-to-date ... It is clearly written and illustrated, and will appeal to professional biochemists interested in enzymes. Natural Product Reports


Contents

1. Principles of enzyme assay and kinetic studies; 2. Photometric assays; 3. Radiometric assays; 4. High performance liquid chromatography assays; 5. Electrochemical assays: the oxygen electrode; 6. Electrochemical assays: the nitric oxide electrode; 7. Electrochemical assays: the pH-stat; 8. Enzyme assays after gel electrophoresis; 9. Techniques for enzyme extraction; 10. Determination of active site concentration; 11. High throughput screening - considerations for enzyme assays; 12. Statistical analysis of enzyme kinetic data


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