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Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.
- Preface
- Tools and Techniques of Kinetic Analysis
- How do Enzymes Work
- Characterization of Enzyme Activity
- Reversible Enzyme Inhibition
- Irreversible Enzyme Inhibition
- pH Dependence of Enzyme-Catalyzed Reactions
- Two-Substrate Reactions
- Multisite and Cooperative Enzymes
- Immobilized Enzymes
- Interfacial Enzymes
- Transient Phases of Enzymatic Reactions
- Characterization of Enzyme Stability
- Mechanism-Based Inhibition (Leslie J. Copp)
- Putting Kinetic Principles into Practice (Kirk L. Parkin)
- Use of Enzyme Kinetic Data in the Study of Structure-Function Relationships of Proteins (Takuji Tanaka and Rickey Y. Yada)
- Bibliography
- Index
A. G. Marangoni, PhD, is Professor and Canada Research Chair in Food and Soft Materials in the Department of Food Science at the University of Guelph, Ontario, Canada.
" [...] a useful book as an introduction to the topic [...] complements existing authorative treatments in a concise way [...] "
- Journal of The Science of Food and Agriculture, Vol.85, No.1, 15 January 2005
" [...] a concise introduction to enzyme kinetics [...] recommended [...] "
- Choice, Vol. 40, No. 9, May 2003
" [...] extremely useful [...] particularly [...] the applications and practical examples to demonstrate the mathematical handling of experimental data [...] "
- Clinical Chemistry, Vol. 49, No. 5, 2003
" [...] a valuable companion for everyone seeking a quick overview of the theory of enzyme kinetics."
- Angewandte Chemie International Edition, Vol. 42, 2003
" [...] a useful manual for the experienced researcher and the developing enzymologist."
Journal of Food Quality, Vol. 26
" [...] an innovative approach to the field of enzyme kinetic modeling, model transformation, and model analysis [...] a valuable reference for practitioners in this field."
- SIM News, Vol. 53, No. 3, May/June 2003
" [...] presents a new way of looking at an old subject [...]
- Angewandte Chemie, 2003
" [...] the approach is quite mathematical and will appeal to the reader versed in mathematics and kinetics [...] "
- ChemBioChem, Vol 4(5), 2003
