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About this book
About this book
Fully revised and updated primer for teaching the essentials of plant tissue culture.
Part I. Background Information: 1. Culture of plant cells, tissues, and organs; 2. Laboratory facilities; 3. Aseptic techniques; 4. Media composition and preparation; Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus; 6. Organogenesis; 7. Cell suspensions; 8. Somatic embryogenesis; Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots; 10. Micropropagation by bud proliferation; 11. Anther and pollen cultures; Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements; 13. Isolation and culture of protoplasts; 14. Protoplast fusion and somatic hybridization; Part V. Supplementary Topics: 15. Cryopreservation of germplasm; 16. Production of secondary metabolites; 17. Quantitation of procedures; 18. Formulations of tissue culture media; 19. Commercial sources of supplies; Author index; Subject index.
Textbook Handbook / Manual
256 pages, B/w photos, figs, tabs
'This well thought out book does considerably more than is claimed in the title. It not only takes the reader through a graded series of experimental protocols covering the major techniques used in plant tissue culture, it also provides a good introductory review of each topic.' Trends in Biochemical Sciences '... the authors have written ... a text based on their extensive experience ... directed in the first instance towards students, these topics will prove [to be] of immense value to a much wider range of readers, whatever their previous knowledge.' Cellular and Molecular Biology '...comprehensive and yet concise...Invaluable to all those interested or involved in the area of plant tissue culture and commends itself as an important source of information about techniques used in a wide range of research and teaching institutions as well as in commercial companies. This excellent book achieves much more than its title suggests.' G. Ben-Hod, Israel Journal of Plant Science '... a worthy text and an excellent addition to any plant science laboratory.' John Love, British Society for Developmental Biology Newsletter