Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology.
Topics described in this manual include:
FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.
From the contents (Parts A - G):The Standard FISH Procedure - an Introduction; FISH on Native, Human Tissues; FISH on Archival, Human Tissues; Special FISH Techniques (Fiber-FISH, PNA-FISH, CO-FISH, NU-FISH); FISH in Zoology and Virology; Multicolor FISH Applications; Not FISH but Almost; Appendix: Internet Sites Related to Cytogenetics and Molecular Cytogenetics.
From the reviews:
"Ever wondered how to catch your FISH a ] in the dark pools of cellular biology? Then a ] FISH technology might be a welcome addition to your library. Thirty-six chapters on 485 pages compile the latest protocols and background information on fluorescence in situ hybridization (or FISH) a ] . Every chapter is illustrated by several schematic drawings a ] . The manual is completed by a subject index a ] . The book is certainly clear a ] providing valuable protocols for both beginning FISHers and for FISH experts." (Dr. Jan Roelof Van der Meer, BioWorld, Issue 6, 2002)