Volume 2 focuses on hyphomycetes, a group of asexual fungi in which conidia are not formed within complex conidiomata, such as pycnidia. Most of the species covered belong to the Ascomycota, with a few representatives from the Basidiomycota. In total, 296 species and one variety, across 101 genera, are described, illustrated, and keyed out, with distribution maps provided for most species. Of these, 271 species and one variety are considered obligate lichenicolous fungi, while an additional 25 species are facultatively or doubtfully lichenicolous. This volume introduces four new genera (Gyalectiphila, Llanorella, Pseudophaeoisaria, and Stipititheca), 53 new species, 11 new combinations, two new lectotypifications, and 13 new synonymies. Phylogenetic trees are included for genera where reliable molecular data for lichenicolous asexual species are available.