Fluorescence Microscopy From Principles to Biological Applications

About this book

Fluorescence Microscopy is the first title on the topic designed specifically to allow students and researchers with no background in physics to understand novel light microscopy techniques. Each chapter in Fluorescence Microscopy is written by a renowned expert with many contributions by pioneers in the field, but the editors have ensured that all commonly used methods are covered and that each chapter is comprehensible for non-experts and conforms to the same easily legible standard and style. A companion website with additional examples and video material makes this a valuable teaching resource.

Contents

Preface

- Introduction to Optics and Photophysics (Rainer Heintzmann)
- Principles of Light Microscopy (Ulrich Kubitscheck)
- Fluorescence Microscopy (Jurek W. Dobrucki)
- Fluorescence Labeling (G. Ulrich Nienhaus, Karin Nienhaus)
- Confocal Microscopy (Nikolaus Naredi-Rainer, Jens Prescher, Achim Hartschuh, Don C. Lamb)
- Fluorescence Photobleaching and Photoactivation Techniques (Reiner Peters)
- Förster Resonance Energy Transfer and Fluorescence Lifetime Imaging (Fred S. Wouters)
- Single molecule microscopy in the live sciences (Markus Axmann, Josef Madl, Gerhard J. Schütz)
- Super-Resolution Microscopy Interference and Pattern Techniques (Gerrit Best, Roman Amberger, Christoph Cremer)
- STED Microscopy (Trevis J. Gould, Patrina A. Pellet, Joerg Bewersdorf)

Appendix: Practical guide to optical alignment (Rainer Heintzmann)

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Biography

Ulrich Kubitscheck is the department head of Biophysical Chemistry at the Institute of Physical and Theoretical Chemistry at the Rheinische Friedrich-Wilhelms-Universitat in Bonn, Germany. Having obtained his academic degrees from the University of Bremen, he spent his career working a The Weizmann Institute of Science and the Institute of Medical Physics and Biophysics of the Westfalische Wilhelms-Universitat Munster before taking up his present appointment at Bonn University. Professor Kubitscheck develops single molecule imaging techniques, has authored over 60 scientific publications and has extensive experience in teaching courses on physical chemistry, biophysics and quantitative microscopy.

Reiner Peters is Visiting Professor at the Rockefeller University in New York, USA. Having obtained his academic degrees from the Freie Universitat, Berlin, Germany, he worked at Max-Planck-Institutes in Gottingen and Frankfurt and was a professor at the Institute of Medical Physics and Biophysics of the Westfalische Wilhelms-Universitat Munster, Germany, before moving to the Rockefeller University in New York, USA. He has invented the fluorescence photobleaching technique and other quantitative light microscopic techniques and published in the field of light microscopy and cellular biophysics.