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The latest edition of this highly successful textbook introduces the key techniques and concepts involved in cloning genes and in studying their expression and variation.
The new edition features:
- Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics.
- Clear, two-colour diagrams throughout.
- A dedicated website including all figures.
Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.
1 From Genes to Genomes 1
2 How to Clone a Gene 25
3 Genomic and cDNA Libraries 75
4 Polymerase Chain Reaction (PCR) 109
5 Sequencing a Cloned Gene 131
6 Analysis of Gene Expression 169
7 Products from Native and Manipulated Cloned Genes 189
8 Genomic Analysis 229
9 Analysis of Genetic Variation 275
10 Post-Genomic Analysis 305
11 Modifying Organisms: Transgenics 327
"This third edition is absolutely necessary to incorporate the recent advances, such as genome sequencing, polymerase chain reaction, and microarray technology, in this field."
- Doody's, 19 October 2012