Fundamentals of Fluorescence Microscopy starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, Fundamentals of Fluorescence Microscopy approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus.
The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. Fundamentals of Fluorescence Microscopy is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
1 Ray Optics, Wave Optics and Imaging System Designing
2 Basics of Electromagnetic Theory for Fluorescence Microscopy
3 Electric Field Effects in Optical Microscopy Systems
4 Quantum Description of Radiation Field and Optical Microscopy
5 Molecular Physics of Fluorescence Markers
6 Basics of Fluorescence and Photophysics
7 General Fluorescence Imaging Techniques
8 Multiphoton Fluorescence Microscopy
9 Super Resolution Fluorescence Microscopy
10 Image Reconstruction Methodologies for Fluorescence Microscopy
11 Future Prospective of Fluorescence Microscopy
Alberto Diaspro is Professor of Physics, Biophysics and Advanced Microscopy Methods at the University of Genoa in the Applied Physics Area, IIT Director and PI, and member of the Medical Nanotechnology PhD Advisory Committee (University of Milan). Further, he heads a research group at IFOM - FIRC Oncology Institute, Milan, has scientific collaborations with the Institute of Biophysics of the National Research Council (CNR), and founded LAMBS in 2003 (Laboratory for Advanced Microscopy, Bioimaging and Spectroscopy, www.lambs.it). He has been President of EBSA (European Biophysical Societies' Association, www.ebsa.org) (2009-2011), President of OWLS (Optics with Life Sciences) (2010-2012) and member of the International Relations Committee of the Biophysical Society, www.biophyscs.org. Also, he is active member of the Biophysical Society, EBSA, SPIE, OSA, IEEE, OWLS (Optics Within Life Sciences of the International Commission for Optics, ICO), FOM (Focus on Microscopy) and SIF (Italian Physical Society, www.sif.it). He has written more than 200 ISI publications.
Partha Pratim Mondal is an assistant professor at the Indian Institute of Science in the department of Instrumentation and Applied Physics. He received his Ph.D. in Physics from the Indian Institute of Science, Bangalore, India in 2005. He has been a researcher at the MIT, Cambridge, MA, USA and at the universities of Genova, Italy and also was a ICTP Fellow, Trieste, Italy. He has more than 30 journal publications to his name and 18 contributions to conference proceedings. Currently, he is Associate Editor for Scientific Reports (Nature Publishing) , Academic Editor to the AIP Advances journal and an editorial board member to Microscopy, Research and Technique (Wiley).