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Guide to Opisthobranchs of Andaman and Nicobar Islands updates the available knowledge of the ophisthobranchiate fauna of the Andaman and Nicobar Islands, India. The update is based on a series of surveys of 15 areas, including 27 islands during April to November 2009. A total of 81 species are described, including morphological description, a mention of similar species, distribution, and additional remarks. Identification keys are included for relevant genera.