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This book is aimed at providing a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function. The techniques have wide applications in biology, and underpin some aspects of nanotechnology and quantum information processing.
1. Introduction; 2. Single Molecule Fluorescence Techniques; 3. Single Molecule Fluorescence Instrumentation; 4. Preparation of samples for single molecule fluorescence spectroscopy; 5. Fluorescence spectroscopy of freely diffusing single molecules: examples; 6. Fluorescence spectroscopy of immobilised single molecules: examples; 7. The outlook for single molecule fluorescence measurements