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About this book
About this book
Suggests cost-effective, simple, and sensitive immunological techniques to assess plant health and food safety for the production of desirable foods, feeds, and timbers.
Foreword (M. S. Swaminathan) Preface Acknowledgments List of Abbreviations PART I: PRINCIPLES AND TECHNIQUES Chapter 1. Introduction Agroecosystems and Microbes Plant Health Management Applications of Immunology Summary Chapter 2. Principles of Immunological Reactions Antigens Antibodies Summary Chapter 3. Preparation of Antisera Polyclonal Antisera Monoclonal Antisera Phage-Displayed Recombinant Antibodies Purification of Immunoglobulins and Antibody Fragments Summary Chapter 4. Basic Immunodetection Techniques Precipitation and Agglutination Reactions Immunodiffusion Tests Immunoelectrophoresis Labeled Antibody Techniques Immunoelectron Microscopy Immunocapture-Reverse Transcription-Polymerase Chain Reaction (IC-RT-PCR) Technique Immunocapillary Zone Electrophoresis (I-CZE) Summary PART II: STRUCTURE AND CONSTITUENTS OF HEALTHY PLANTS Chapter 5. Structure and Functions of Plant Components Components of Plant Cells Organization of Tissues of the Plant Body Summary Chapter 6. Biochemical Constituents of Plants Carbohydrates Proteins Nucleic Acids Summary Appendix: Assay of Polygalacturonase-Inhibiting Protein (PGIP) by Immunoblotting Technique PART III: PLANT-STRESS INTERACTIONS Chapter 7. Plant-Abiotic Stress Interactions Salt Stress Water Stress Temperature Stress Summary Chapter 8. Plant-Fungal Pathogen Interactions Immunodiagnostic Techniques Enzyme-Linked Immunosorbent Assay (ELISA) Phases of Fungal Disease Development Summary Appendix: Immunological Protocols for Detection of Fungal Pathogens Chapter 9. Plant-Bacterial Pathogen Interactions Immunodiagnostic Techniques for Bacterial Pathogens Immunodiagnosis of Phytoplasmal Diseases Phases of Development of Bacterial Diseases Summary Appendix: Diagnostic Techniques for the Detection of Bacterial Pathogens Chapter 10. Plant-Viral Pathogen Interactions Immunodiagnostic Techniques for Plant Viruses Virus Disease Development Summary Appendix: Techniques for Detection of Viral Pathogens PART IV: APPROACHES FOR PLANT HEALTH MANAGEMENT Chapter 11. Improvement of Host Plant Resistance Through Genetic Manipulation Breeding for Resistance to Crop Diseases Engineering Resistance to Crop Diseases Summary Appendix: Quantification of Infection and Detection by Immunological Techniques Chapter 12. Induction of Disease Resistance and Production of Disease-Free Plants Induction of Resistance to Microbial Plant Pathogens Cross Protection Production of Disease-Free Plants Summary Appendix: Protocol for Polyclonal Antiserum Production PART V: ASSESSMENT OF FOOD SAFETY Chapter 13. Mycotoxins and Food Safety Mycotoxin Contamination Detection of Mycotoxins Management of Mycotoxin Hazards Summary Appendix: Protocols for the Detection of Mycotoxins Chapter 14. Chemical Residues and Food Safety Detection of Fungicides Detection of Pesticides Applied Against Insect Vectors Detection of Predation of Crop Pests Summary Appendix: Immunoassay Protocols References Index