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The techniques for establishing and maintaining invertebrate tissues and cells in culture remain difficult due to the diversity of invertebrates and their structural and physiological characteristics. Research involving invertebrate cell cultures continues to increase, although the number of cell lines used is still limited.
This manual gives detailed descriptions of the technical procedures for the establishment of primary invertebrate cell cultures in vitro. Nutritional requirements, culture media, and species-specific methods for both cell and organ cultures as well as useful techniques for studies on cultured cells are described. The Appendix lists established cell lines available for research with information on the composition of their physiological and nutrient solutions. This comprehensive manual, the first of its kind, is a valuable reference for investigators working with invertebrate cell cultures in academia and industry.
Part I General Methods: Facilities and Equipment; Basic Information and Overview; Preparation of Media; General Cell Culture Methods. Part II Methods for Setting Up Primary Cultures Specific to Animal Groups: Insecta (Lepidoptera); Insecta (Diptera); Insecta (Coleoptera); Insecta (Hemiptera); Insecta (Hymenoptera); Insecta (Blattaria); Insecta (Orthoptera); Arthropoda Other than Insecta; Prochordata; Echinodermata; Mollusca; Annelida; Nematoda; Plathelminthes; Coelenterata; Porifera; Part III Organ cultures: Insecta; Arthropoda other than Insecta; Prochordata; Echinodermata; Mollusca; Annelida; Nematoda; Plathelminthes; Coelenterata; Porifera. Part IV Related Techniques: Cell Cloning; Karyotype Analyses; Identification of Cell Lines; Viability of Cells; Viable Cell Enumeration; Growth Rate; Microscope Photography; Virus Inoculation-Plaque Assay; Examination of Drug Effects; Cell Fusion; Gene technology; Large Scale Cell Cultures; Appendices: Composition of Salt Solutions and Culture Meda - Salt Solutions, Culture Media; List of Invertebrate Continuous Cell Lines Reported; Commercial Products of Media and List of Suppliers - Commercial Products of Media, List of Suppliers.