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Academic & Professional Books  Organismal to Molecular Biology  Microbiology

Laboratory Fundamentals Of Microbiology

By: Jeffrey C Pommerville(Author)
572 pages
Publisher: Jones & Bartlett
Laboratory Fundamentals Of Microbiology
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  • Laboratory Fundamentals Of Microbiology ISBN: 9781284100976 Edition: 11 Paperback Jul 2017 Out of Print #238309
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About this book

This popular lab manual that for years has taught students the basics of working in a lab and how to safely handle microorganisms, has been completely redesigned and updated. All labs have been expanded and reorganized to fit neatly into new sections of the lab, such as "Fungi", "Viruses", or "Biotechnology", and are presented from easiest to most challenging. New introductions for each section provide the appropriate context for the labs and connects the lab concepts to the core text, Fundamentals of Microbiology, Eleventh Edition. The most exciting update are the new videos available with the Lab Manual that teach common lab skills that tie to the labs in the manual. Students will clearly see how to work safely in the lab setting, how to use operating equipment, how to swab cultures, and many more lab skills. New photos and micrograph examples and new exercises and assessments only further enhance the eleventh edition of Laboratory Fundamentals of Microbiology, making it the easy choice for microbiology lab courses.


Part  I  Laboratory Safety
      Lab  1  Safety overview: Biohazards
      Lab  2  Best practices in safety
      Lab  3  Student safety contract
Part  II  Laboratory Techniques and Skills
      Lab  4  Culture transfer techniques
      Lab  5  Pure culture techniques
      Lab  6  Pour plate technique
      Lab  7  Solution Transfer
      Lab  8  Serial Dilutions
Part  III  Microscopy
      Lab  9  Parts of the microscope
      Lab  10  Observation of prepared slides
Part  IV  Bacterial Staining Techniques
      Lab  11  Preparation of a bacterial smear
      Lab  12  Simple stain technique
      Lab  13  The negative stain technique
      Lab  14  The Gram stain technique
      Lab  15  Bacterial structures – Spore stain
      Lab  16  Bacterial structures – Capsule stain
      Lab  17  Bacterial structures – Bacterial motility, A. Hanging drop B. Motility test agar technique
Part  V  Viruses and Eukaryotic Microorganisms
      Lab  18  The effect of bacteriophages on bacteria
      Lab  19  Plaque formation
      Lab  20  Identification of bacteriophages from sewage
      Lab  21  Fungi: Molds
      Lab  22  Fungi: Yeasts
      Lab  23  Protozoa and multicellular parasites
Part  VI  Control of Microorganisms
      Lab  24  The effect of physical agents on bacteria: Heat tolerance
      Lab  25  The effect of physical agents on bacteria: Ultraviolet light
      Lab  26  The effect of physical agents on bacteria: Drying
      Lab  27  The effect of physical agents on bacteria: Other physical agents, A. Incineration B. Osmotic pressure C. Cold temperature D. Autoclaving
      Lab  28  The effect of chemical agents on bacteria: Antiseptics and disinfectants
      Lab  29  The effect of chemical agents on bacteria: Metals
      Lab  30  The effect of chemical agents on bacteria: Other chemical substances
      Lab  31  Effect of lysozyme on bacteria
      Lab  32  Evaluation of disinfectants and antiseptics: Effectiveness of disinfectants on inanimate objects
      Lab  33  Effectiveness of disinfectants on hand washing and the skin surface
      Lab  34  Mouthwashes and oral bacteria
      Lab  35  The effect of antibiotics on bacteria
Part  VII  Measuring Population Growth
      Lab  36  Estimating the number of bacterial cells in a population, • The Petroff-Hauser Chamber, • The Standard Plate Count
      Lab  37  Microbial growth: Analysis of a bacterial growth curve, • Bacterial growth dynamics, • Plotting a growth curve and determining the generation time
Part  VIII  Medical Microbiology
      Lab  38  The genus Mycobacterium: Colony and cellular morphology
      Lab  39  The genus Mycobacterium: Acid-fast stain technique
      Lab  40  The genus Mycobacterium: Cold acid-fast stain technique
      Lab  41  The genus Streptococcus: Streptococci from the upper respiratory tract
      Lab  42  The genus Streptococcus: Streptococci from the oral cavity
      Lab  43  The genus Neisseria
      Lab  44  The genus Staphylococcus: Isolation of Staphylococci
      Lab  45  The genus Staphylococcus: Differentiation between Staphylococcal species
      Lab  46  The enteric bacteria: Isolation of enteric bacteria
      Lab  47  The enteric bacteria: Differentiation of enteric bacteria on TSI agar
      Lab  48  The enteric bacteria: The IMViC series, A. Indole test B. Methyl red test C. Voges-Proskauer test D. Citrate test
      Lab  49  The enteric bacteria: Rapid identification of enteric bacteria
      Lab  50  The genus Bacillus
      Lab  51  The genus Clostridium
      Lab  52  The genus Lactobacillus: Isolation of Lactobacilli
      Lab  53  The genus Lactobacillus: Caries susceptibility test
Part  IX  Identification of a Bacterial Unknown
      Lab  54  Bacterial structural characteristics
      Lab  55  Bacterial culture characteristics
      Lab  56  Biochemical characteristics of bacteria: Carbohydrate fermentation
      Lab  57  Biochemical characteristics of bacteria: Starch digestion
      Lab  58  Biochemical characteristics of bacteria: Catalase production
      Lab  59  Biochemical characteristics of bacteria: DNA digestion
      Lab  60  Biochemical characteristics of bacteria: Hydrogen sulfate production
      Lab  61  Biochemical characteristics of bacteria: IMViC series
      Lab  62  Biochemical characteristics of bacteria: Urea digestion
      Lab  63  Biochemical characteristics of bacteria: Lipid digestion
      Lab  64  Biochemical characteristics of bacteria: Casein digestion
Part  X  Bacterial Genetics
      Lab  65  Bacterial Genetics: Mutations and the Ames test – Gradient plate technique
      Lab  66  Bacterial Genetics: Mutations and the Ames test – Mutagenic effect of ultraviolet light
      Lab  67  Bacterial Genetics: Mutations and the Ames test – DNA repair
      Lab  68  Bacterial Genetics: Mutations and the Ames test – The Ames test
      Lab  69  Bacterial conjugation
      Lab  70  Bacterial transformation
Part  XI  Immunology
      Lab  71  Serology: Agglutination techniques and blood cell identification – Slide agglutination
      Lab  72  Serology: Agglutination techniques and blood cell identification – Tube agglutination
      Lab  73  Serology: Agglutination techniques and blood cell identification – Hemmagglutination: Determination of blood type
      Lab  74  Serology: Agglutination techniques and blood cell identification – Blood smear
      Lab  75  ELISA test
Part  XII  Public Health and Environmental Microbiology
      Lab  76  A simulated epidemic: Microbial transmission via fomites
      Lab  77  A microbial hunt
      Lab  78  Transmission of microorganisms through toilet paper
      Lab  79  Disinfection of Drinking Water
      Lab  80  Microbiology of foods: Food preservation with salt and garlic
      Lab  81  Microbiology of foods: Standard plate count of food products
      Lab  82  Microbiology of foods: Fermentation of wine and beer
      Lab  83  Microbiology of milk and dairy products: Standard plate count of milk
      Lab  84  Microbiology of milk and dairy products: Coliform plate count of milk
      Lab  85  Microbiology of milk and dairy products: Methylene blue reduction test
      Lab  86  Microbiology of milk and dairy products: Preparation of cheese
      Lab  87  Microbiology of milk and dairy products: Preparation of yogurt
      Lab  88  Microbiology of milk and dairy products: The natural bacterial content of milk
      Lab  89  Microbiology of water: Presumptive test by the MPN method
      Lab  90  Microbiology of water: Confirmed test
      Lab  91  Microbiology of water: Completed test
      Lab  92  Microbiology of water: Membrane filter technique
      Lab  93  Microbiology of water: Preparation of a biofilm
      Lab  94  Microbiology of soil: Isolation of Rhizobium from legume roots
      Lab  95  Microbiology of soil: Ammonification of soil microorganisms
      Lab  96  Microbiology of soil: Isolation of Streptomyces from soil
      Lab  97  Microbiology of soil: Antibiotic production by Streptomyces
      Lab  98  Microbiology of soil: Plate count of soil bacteria
      Lab  99  Microbiology of soil: Microbiology ecology in the soil – the Winogradsky column

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Jeffrey Pommerville is Professor of Biology and Microbiology at Glendale Community College (GCC) in Glendale, Arizona, where he teaches microbiology and introductory biology. He also has served as the Course Assessment Coordinator for the Biology Department at GCC. Before coming to GCC, he was on the biology faculty at Texas A&M University. Dr. Pommerville received his BS and PhD from the University of California-Santa Barbara. Since coming to GCC, Dr. Pommerville has served as principal investigator on several NSF grants, including Systemic Reform in Science (SyRIS) that developed a strategy to reform and integrate the introductory sciences. He is the author of more than 50 research and education research papers, and was the Perspectives Editor for the Journal of Microbiology and Biology Education, a peer-reviewed journal published by the American Society for Microbiology (ASM). Dr. Pommerville has received numerous professional honors, including: the 2008 Carski Foundation Distinguished Undergraduate Teaching Award from ASM, the Golden Microscope Award from the Leukemia and Lymphoma Society, the Ohaus Award for Innovations in Science Teaching from the National Science Teachers Association, and was one of the first recipients of a Faculty Distinguished Teaching Award from his home institution. He is a member of AAAS, NSTA, NABT, and, of course ASM. Dr. Pommerville is a past co-chair for the ASM’s Conference for Undergraduate Educators as well as a past chair of Division W of ASM.

Dr. Pommerville also is author of Fundamentals of Microbiology (Body Systems Edition), Third Edition (2016), Alcamo’s Microbes and Society, Fourth edition (2016), as well as Encounters in Microbiology, Volume 1 and Volume 2 (2009), and Guide to Infectious Diseases by Body System, Second Edition (2012).

By: Jeffrey C Pommerville(Author)
572 pages
Publisher: Jones & Bartlett
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