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Academic & Professional Books  Organismal to Molecular Biology  Genetics & Genomics

Manipulating the Mouse Embryo A Laboratory Manual

Handbook / Manual
By: Richard Behringer(Author), Marina Gertsenstein(Author), Kristina Nagy(Author), Andras Nagy(Author)
814 pages
Manipulating the Mouse Embryo
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  • Manipulating the Mouse Embryo ISBN: 9781936113019 Edition: 4 Paperback Nov 2013 Not in stock: Usually dispatched within 1-2 weeks
  • Manipulating the Mouse Embryo ISBN: 9781936113002 Edition: 4 Hardback Oct 2013 Out of Print #210653
Selected version: £103.00
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About this book

This fourth edition of Manipulating the Mouse Embryo is once again the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Its preeminent authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy have reorganized and updated this edition to include new information and protocols on:
- generation of induced pluripotent stem cells
- RNA microinjections
- lentiviral microinjections and infection
- assisted reproduction techniques for sperm and embryo cryopreservation
- isolation, generation, and transplantation of spermatogonial stem cell lines
- in utero electroporation of gene constructs into postimplantation embryos
- vibratome sectioning of live and fixed tissues for imaging thick tissue sections
- whole-mount fluorescent staining methods for three-dimensional visualization

Recombinant DNA techniques and methods for studies of mouse embryonic development have been updated from previous editions, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of this classic work appeared more than 20 years ago, with authors that included Brigid Hogan, Rosa Beddington, Frank Costantini, and Elizabeth Lacy. The field's technological sophistication has grown exponentially but Manipulating the Mouse Embryo remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.

Customer Reviews

Handbook / Manual
By: Richard Behringer(Author), Marina Gertsenstein(Author), Kristina Nagy(Author), Andras Nagy(Author)
814 pages
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