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Mass Spectrometry in Structural Biology and Biophysics Architecture, Dynamics and Interaction of Biomolecules

Handbook / Manual
By: Igor A Kaltashov, Stephen J Eyles, Dominic M Desiderio and Nico Nibbering
316 pages, illustrations
Mass Spectrometry in Structural Biology and Biophysics
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  • Mass Spectrometry in Structural Biology and Biophysics ISBN: 9780470937792 Edition: 2 Hardback Apr 2012 Usually dispatched within 4 days
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About this book

The use of mass spectrometry (MS) to study the architecture and dynamics of proteins is increasingly common within the biophysical community, and "Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics, and Interaction of Biomolecules", Second Edition provides readers with detailed, systematic coverage of the current state of the art.

Offering an unrivalled overview of modern MS-based armamentarium that can be used to solve the most challenging problems in biophysics, structural biology, and biopharmaceuticals, the book is a practical guide to understanding the role of MS techniques in biophysical research. Designed to meet the needs of both academic and industrial researchers, it makes mass spectrometry accessible to professionals in a range of fields, including biopharmaceuticals.

This new edition has been significantly expanded and updated to include the most recent experimental methodologies and techniques, MS applications in biophysics and structural biology, methods for studying higher order structure and dynamics of proteins, an examination of other biopolymers and synthetic polymers, such as nucleic acids and oligosaccharides, and much more.

Featuring high-quality illustrations that illuminate the concepts described in the text, as well as extensive references that enable the reader to pursue further study, Mass Spectrometry in Structural Biology and Biophysics is an indispensable resource for researchers and graduate students working in biophysics, structural biology, protein chemistry, and related fields.

Contents

Preface to the Second Edition xi
Preface to the First Edition xiii

1 General Overview of Basic Concepts in Molecular Biophysics 1
2 Overview of Traditional Experimental Arsenal to Study Biomolecular Structure and Dynamics 26
3 Overview of Biological Mass Spectrometry 52
4 Mass Spectrometry Based Approaches to Study Biomolecular Higher Order Structure 89
5 Mass Spectrometry Based Approaches to Study Biomolecular Dynamics: Equilibrium Intermediates 127
6 Kinetic Studies By Mass Spectrometry 160
7 Protein Interactions: A Closer Look at the Structure--Dynamics--Function Triad 186
8 Other Biopolymers and Synthetic Polymers of Biological Interest 212
9 Mass Spectrometry on the Frontiers of Molecular Biophysics and Structural Biology: Perspectives and Challenges 239

Appendix: Physics of Electrospray 279
Index 285

Customer Reviews

Biography

IGOR A. KALTASHOV, PHD, is a Professor in the Department of Chemistry at the University of Massachusetts Amherst.

STEPHEN J. EYLES, PHD, is a Senior Lecturer in the Department of Biochemistry and Molecular Biology, and the Director of the Mass Spectrometry Center at the University of Massachusetts Amherst.

Handbook / Manual
By: Igor A Kaltashov, Stephen J Eyles, Dominic M Desiderio and Nico Nibbering
316 pages, illustrations
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