Never before has it been so critical for lab workers to possess the proper tools and methodologies necessary to determine the structure, function, and expression of the corresponding proteins encoded in the genome. Mulhardt's Molecular Biology and Genomics helps aid in this daunting task by providing the reader with tips and tricks for more successful lab experiments. This strategic lab guide explores the current methodological variety of molecular biology and genomics in a simple manner, addressing the assets and drawbacks as well as critical points. It also provides short and precise summaries of routine procedures as well as listings of the advantages and disadvantages of alternative methods.
1. What the Heck is 'Molecular Biology' Anyway?
2. Some Fundamental Methods
3. The Tools
4. The Polymerase Chain Reaction (PCR)
6. The Cloning of DNA Fragments
7. How to track down DNA
8. DNA Analysis
9. Investigation of the Function of DNA Sequences
10. The Computer and You
11. Suggestions for Career Planning or: The Mini 'Machiavelli' for Young Researchers
Praise for the German edition:
"This lab book illustrates ways out of experimental dead ends and teaches a feeling for doing the right experiment at the right time."
– Swiss Laboratory Magazine
"With innumerable tips to complete basic knowledge, Mulhardt paves the cumbersome ways from nucleic acids to their translation to gene expression, gene therapy and more."
– Laboratory Journal
"It's no miracle that this book has had a major sales impact."
– Food And Biotechnology
"The book reveals the laboratory secrets that are usually learned the hard way, like how not to let agarose boil over in the microwave. Besides protocols and buffer recipes, the book gives advice for setting realisitc goals and eventually becoming an independent scientist , including these encouraging words: 'The experiment will be successful, as long as you are strong enough to put up with six months of the deepest depressioin.' I wish I had this book when I started graduate school."
– Wendy Chao, in The Scientist, April 1, 2007