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Provides both a theoretical and a methodological introduction to various key molecular techniques used in microbial ecology, focusing particularly on nucleic acid-based methodologies.
1. Nucleic Acid Extraction From Environmental Samples. 2. Prokaryotic Systematics: PCR And Sequence Analysis Of Amplified 16S rRNA Genes. 3. DNA Fingerprinting Of Microbial Communities. 4. Molecular Typing Of Environmental Isolates. 5. RT-PCR And mRNA Expression Analysis Of Functional Genes. 6.Quantitative Real-Time PCR. 7. Stable Isotope Probing. 8. Applications Of Nucleic Acid Hybridisation In Microbial Ecology. 9. Fluorescence in situ Hybridisation For The Detection Of Prokaryotes. 10. Lessons From The Genomes: Microbial Ecology And Genomics. 11. Metagenomic Libraries From Uncultured Microorganisms. 12. A Molecular Toolbox For Bacterial Ecologists: PCR Primers For Functional Gene Analysis. 13. Molecular Detection Of Fungal Communities In Soil. 14. Environmental Assessment: Bioreporter Systems. 15. Bioinformatics And Web Resources For The Microbial Ecologist.
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Dr A. Mark Osborn-Department of Animal & Plant Sciences, University of Sheffield
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