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This book lays out numerous simple techniques for growing and carrying out experiments with many varieties of neurons. Subjects include peripheral and central neurons from vertebrate and invertebrate sources, as well as neuron-like cell lines. It also explains recent advances in our ability to introduce exogenous proteins and genes to neurons in culture. Procedures for successful protein infiltration, biolistic transfection, electroporation, and viral transgenic methods in neurons are also presented. It contains culture methodology for more than a dozen types of CNS and PNS neurons. It includes most recent and reliable techniques from expert practitioners for specific experimental applications. It addresses the latest strategies for transfecting neurons.