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About this book
About this book
Plant cell culture is an essential methodology in plant sciences, with numerous variant techniques depending on the cell type and organism. Plant Cell Culture provides the reader with a concise overview of these techniques, including basic plant biology for cell culture, basic sterile technique and media preparation, specific techniques for various plant cell and tissue types including applications, tissue culture in agriculture, horticulture and forestry and culture for genetic engineering and biotechnology. This book will be an essential addition to any plant science laboratory's bookshelf.
Ch 1. An Introduction to Plant Cell and Tissue Culture. Plant cell and tissue culture. Industrial-scale plant cell culture. Plant tissue culture, plant breeding and crop improvement. Plant tissue culture and plant genetic engineering. Ch 2. Basic Plant Biology for Cell Culture. Tissues and organs. Plant tissues. Meristem. Plant reproductive tissues. The embryo and embryogenesis. Development of tissues. Protoplasts. Media, nutrients and requirements for growth. Ch 3. Tissue Culture in Agriculture, Horticulture and Forestry. Introduction. Micropropagation. Plant breeding. Biodiversity and conservation of germplasm. Ch 4. Tissue Culture in Genetic Engineering and Biotechnology. Introduction. Genetic Engineering. Applications of plant genetic engineering. Ch 5. Culture facilities, Sterile Technique and Media Preparation. Introduction. The basic laboratory layout and equipment. Sterilization. Media preparation. Contamination. Disposing of contaminated waste. Safety in the laboratory. Ch 6. Callus Cultures. Introduction. Origin of callus. Types of callus. Role of callus in embryogenesis, organogenesis and cell culture. Initiation and establishment of callus cultures. Monitoring the growth of callus. Genetic transformation of callus. Ch 7. Cell Suspension Cultures. Introduction. Initiation of cell suspension cultures. Maintenance of cell suspension cultures. Growth characteristics of cell suspension cultures. Uses of cell suspension cultures. Ch 8. Protoplast culture. Introduction. Isolation of protoplasts. Protoplast culture. Uses of protoplasts. Ch 9. Haploid Cultures. Introduction. Anther culture. Microspore culture. Intergeneric crosses and embryo rescue. Procedures for the induction of androgenesis. Ch 10. Organ and Embryo Culture. Introduction. Hairy roots. Embryo culture. Minitubers and microtubers. Ch 11. Regeneration of Plants and Micropropagation. Introduction. Regeneration via somatic embryogenesis. Control of embryogenesis. Regeneration via organogenesis. Protocols. Ch 12. Somaclonal Variation. Introduction. Origins and mechanisms of somaclonal variation. Methods of assessing somaclonal variation in regenerated plants. Somaclonal variation as a technique for crop improvement. Ch 13. Bacterial Culture in the Plant Cell Culture Laboratory. Introduction. Facilities for bacterial culture. Ch 14. Industrial uses of Plant Cell Culture. Introduction. Plant cells in culture. Apparatus for large-scale plant cultures. Examples of industrial-scale production using cultured cells.Ch 15. Prospects and Future Challenges. Recent developments. The future.