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Since the publication of Plant Genotyping: the DNA Fingerprinting of Plants in 2001, the techniques available for plant DNA analysis have advanced considerably. Recent developments focus on high throughput methods, and generally target single nucleotide polymorphism (SNP) discovery and analysis. SNPs represent the most common form of genetic variation in both plants and animals, and play a key role in revealing the molecular mechanisms underlying traits. Plant Genotyping II: SNP Technology describes some of the import recent developments in this field, with the main focus on SNPs. Contributions cover the discovery, analysis and uses of SNPs, while also examining other approaches to plant genotyping.
1. SNP Discovery in Plants, K J Edwards, R L Poole and G L Barker, all University of Bristol, UK; 2. SNPs and Their Use in Maize, A Rafalski and S Tingey, both DuPont Crop Genetics Research, USA; 3. Rare SNP Discovery with Endonucleases, M J Cross, Southern Cross University, Australia; 4. Sequence Polymorphisms in the Flanking Regions of Microsatellite Markers, G Ablett and R Henry, both Southern Cross University, Australia; 5. SNP Discovery by Ecotilling Using Capillary Electrophoresis, F Eliott, G Cordeiro, P C Bundock and R J Henry, both Southern Cross University, Australia; 6. Genotyping by Allele Specific PCR, D L E Waters, P C Bundock and R J Henry, Southern Cross University, Australia; 7. The MassARRAY[registered] System for Plant Genetics, D Irwin, Sequenom, Australia; 8. Mutation Screening, L Izquierdo, Griffith University, Australia. 9. Nanotechnology: The Future of Cost-Effective Plant Genotyping, J A Pattemore, Southern Cross University, Australia; M Trau, University of Queensland, Australia and R J Henry, Southern Cross University, Australia; 10. Functionally-Associated Molecular Genetic Markers for Temperate Pasture Plant Improvement, J W Forster, N O I Cogan, M P Dobrowolski, G C Spangenberg, K F Smith, all Primary Industries Research, Australia; M G Francki, Department of Agriculture and Food, Western Australia; 11. Genotyping for Rice Eating Qualities, L M T Bradbury, D L E Waters and R J Henry, Southern Cross University, Australia; 12. Towards Universal Loci for Plant Genotyping, T Pacey Miller, Southern Cross University, Australia; 13. DNA Banks as a Resource for SNP Genotyping, N Rice, S Kasem and R Henry, Southern Cross University, Australia; 14. DNA Extraction from Plant Tissue, S Kasem, N Rice and R J Henry, Southern Cross University, Australia; 15. Future Prospects for Plant Genotyping, R J Henry, Southern Cross University, Australia.