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Provides the molecular microbiologist with the most important recipes, and a manual of both classical methods and new tools.
Physical analysis of Chromosome size variations, Colin Dale, Wendy Smith and Howard Ochman; Genetic mapping in Salmonella enterica, Josep Casadesus and Eva M. Camacho; Insertion sequences as genomic markers, Dominique Schneider and Michel Blot; The use of noncoding microsatellite length analysis for bacterial strain typing, David Metzgar; How to amplify easily a desired DNA sequence on the bacterial chromosome, Richard D'Ari and Daniel Vinella; Generalized transduction, Anne Tierauf and Stanley Maloy; Use of conditional-replication, integration and modular CRIM plasmids to make single-copy lacZ fusions, Lu Zhou, Soo-Ki Kim, Larisa Avramova, Kirill A. Datsenko and Barry L. Wanner; Genetic footprinting for bacterial funtional genomics, Scott Walker, Chad Houseweart and Teresa J. Kenney; Gene transfer to plants through bacterial vectors, Bruno Tinland; Quorum sensing - approaches to identify signals and signalling genese, Simon Swift; Transcriptional profiling in bacteria using microarrays, Michael T. Laub and R. Frank Rosenzweig; Transcriptome analysis by macroarrays, Cecile Jourlin-Castelli, Francois Denizot and Philippe Bouloc; Prokaryotic proteomics Cecile Lelong and Thierry Rabilloud; Intein-mediated protein purification, Shaorong Chong and Francine B. Perler; Two-hybrid assay in Escherichia coli, Gustavo Di Lallo, Patrizia Ghelardini and Luciano Paolozzi.