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Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In three topical volumes, it covers the full spectrum of current concepts, methods and application areas.
VOLUME 1: FUNDAMENTALS Historical perspective Structure and function in proteins Protein engineering and dynamics Evolution: Protein engineering in Nature Metagenomics and genome prospection Engineering the reaction environment Patents and legal aspects VOLUME 2: METHODS In silico protein design Rational protein engineering Protein evolution in the laboratory Selection and screening In situ directed evolution Library evaluation Production scale biosynthesis VOLUME 3: APPLICATIONS Antibody engineering Engineering secondary metabolism Pathway engineering and multienzyme systems Engineering thermostability Novel enzyme functions Engineering substrate specificity Introducing unnatural amino acids Glycosylation engineering Inteins in protein engineering Biosensors and regulators Protein folding Plant proteins Biomaterials
Stefan Lutz holds a B. S. degree from the Zurich University of Applied Sciences (Switzerland), and a M.S. degree from the University of Teesside (UK). He then obtained a Ph.D. from the University of Florida and spent three years as a Postdoc with Stephen Benkovic at Pennsylvania State University under a fellowship of the Swiss National Science Foundation. Since 2002 he has been a Chemistry professor at Emory University in Atlanta, Georgia (USA). The research in the Lutz laboratory focuses on the structure-function relationship of proteins through combinatorial protein engineering. Uwe Bornscheuer studied Chemistry at the University of Hannover (Germany), where he obtained a Ph. D. at the Institute of Technical Chemistry. He then spent a postdoctoral year at the University of Nagoya, Japan, before returning to Germany to join the Institute of Technical Biochemistry at the University of Stuttgart. Since 1999 he has been Professor for Biotechnology and Enzyme Catalysis at the University of Greifswald. His main research interest is the application of engineered enzymes in the synthesis of optically active compounds and in lipid modification.
Stefan Lutz and Uwe Bornscheuer did a great job! By convincing many of the key players in the field to contribute to this two volume masterpiece, they managed to assemble basically all aspects of modern protein engineering technology under one roof, that is, between four hard covers. (Angewandte Chemie, February 2010) "The Protein Engineering Handbook provides the first comprehensive overview of the highly cross-disciplinary field of protein engineering that many researchers and students have been eagerly awaiting." (ChemBioChem, August 2009)