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Protein Expression: A Practical Approach

Handbook / Manual

Series: Practical Approach Series Volume: 202

Edited By: David Hames and Steve Higgins

288 pages, 10 b/w illus, 40 figs

Oxford University Press

Paperback | May 1999 | #90320 | ISBN: 0199636230
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £79.99 $106/€90 approx
Hardback | Dec 1999 | #90319 | ISBN: 0199636141
Availability: Usually dispatched within 5 days Details
NHBS Price: £98.99 $131/€112 approx

About this book

Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.

"A laboratory manual compiling methods for monitoring protein expression in all the major in vivo and in vitro eukaryotic systems, including cultured mammalian cells, yeasts, vaculovirus, and Xenopus oocytes and also in prokaryotes in cell-free systems that allow coupled transcription-translation. The step-by-step protocols have been prepared by experienced researchers for graduate students, beginning researchers, and senior scientists."--SciTech Book News


1. Protein expression in mammalian cells; 2. Expression in Xenopus oocytes and cell-free extracts; 3. Expressing cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris; 4. Baculovirus expression systems; 5. Protein synthesis in eukaryotic cell-free systems; 6. Prokaryotic in vivo expression systems; 7. Cell-free coupled transcription-translation systems from Escherischia Coli; 8. Monitoring protein expression

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