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Proteins of the Nucleolus: Regulation, Translocation, & Biomedical Functions contains 14 original review chapters each yielding new, exciting and intriguing data about the emerging understanding of nucleolar structure and function in normal, stressed and diseased cells. The goal of this work is to provide special insight into the nucleolus of the past, present and future, as well its regulation, translocation, and biomedical function. A multitude of topics are introduced and discussed in detail, including nucleologenesis, nucleolar architecture, nucleolar targeting, retention, anchoring, translocation, and the relationship between the nucleolus and cancer.
Proteins of the Nucleolus: Regulation, Translocation, & Biomedical Functions also brings together work from several different species, from human to Drosophila to Dictyostelium and other eukaryotic microbes. The final chapter summarizes some of the issues brought up in the various chapters with a view to future research. This book supports the continued emergence of the nucleolus as a dynamic intranuclear region that oversees a vast diversity of events.
Part I Introduction
Chapter 1 Proteins of the nucleolus: An introduction
1.1 Introduction
1.2 Functional consequences of nuclear and nucleolar architecture (Chapter 2)
1.3 rDNA and nucleologenesis in Drosophila (Chapter 3)
1.4 The nucleolus of Dictyostelium and other lower eukaryotes (Chapter 4)
1.5 Human rDNA genes: Identification of four fractions, their functions and nucleolar location (Chapter 5)
1.6 Chromatin organization and the mammalian nucleolus (Chapter 6)
1.7 Chaperones and multitasking proteins in the nucleolus (Chapter 7)
1.8 Nucleolar localization/retention signals (Chapter 8)
1.9 Nucleolar transport of putative GTPase GNL1 and related proteins (Chapter 9)
1.10 Nucleolar protein anchoring and translocation (Chapter 10)
1.11 The nucleolus as a stress response organelle (Chapter 11)
1.12 The nucleolar aspect of breast cancer (Chapter 12)
1.13 Cysteine proteinase inhibitors in the nucleus and nucleolus in activated macrophages (Chapter 13)
1.14 Nucleolar proteins and cancer: The roles of Aurora A-interacting nucleolar proteins in mitosis and cancer (Chapter 14)
1.15 Nucleolar transplantation and human embryogenesis (Chapter 15)
1.16. Conclusion
Part II The nucleolus and nucleolar proteins
Chapter 2 Functional consequences of nuclear and nucleolar architecture
2.1 General aspects of nuclear architecture
2.2 Nuclear architecture and epigenetic events during replication
2.3 General function and structure of nucleoli
2.4 Conclusions and future directions
2.5 Acknowledgements
2.6 Abbreviations
2.7 References
Chapter 3 rDNA and nucleologenesis in Drosophila
3.1 Introduction
3.2 Nucleolar organizers contain rDNA genes
3.3 The Drosophila rDNA repeat unit
3.4 X-Y chromosome pairing in male meiosis is mediated by the 240bp IGS repeat
3.5 Growth-related expression of Drosophila rDNA
3.6 Centric heterochromatin flanking the NORs
3.7 Endo-replication of Drosophila rDNA
3.8 The bobbed mutation and rDNA magnification
3.9 rDNA compensation
3.10 Early work on R1 and R2 retrotransposons
3.11 Drosophila rDNA and epigenetic effects
3.12 Nucleologenesis in Drosophila melanogaster
3.13 Future endeavors
3.14 Acknowledgements
3.15 Abbreviations
3.16 References
Chapter 4 The nucleolus of Dictyostelium and other lower eukaryotes
4.1 The nucleolus of Dictyostelium
4.1.1 Dictyostelium nucleolar structure during growth
4.2 Nucleolar proteins in Dictyostelium
4.3 The nucleolus in other lower eukaryotes
4.4 Conclusions
4.5 References
Chapter 5 Human rDNA genes: Identification of four fractions, their functions and nucleolar location
5.1 Introduction
5.2 The rDNA copy number in the human genome
5.3 Four rDNA fractions, which differ in their structure, location, and functional activity
5.4 Phenotypical manifestations of genomic dose of active rRNA gene copies
5.5 References
Chapter 6 Chromatin organization and the mammalian nucleolus
6.1 Genome organization principles and nuclear architecture
6.2 Organization of rRNA genes in the mammalian nucleolus
6.3 Chromosomal constitution of the human nucleolus-associated DNA
6.4 Epigenomics of the nucleolus from the proteomics perspective
6.5 Concluding remarks
6.6 References
Chapter 7 Chaperones and multitasking proteins in the nucleolus
7.1 Nucleolar organization, chaperones and multitasking proteins
7.2 Analysis of nucleolar components and functions: New quantitative tools to measure proteins and transcription in the nucleolus
7.3 Targeting chaperones to the nucleolus
7.4 Nucleolar multitasking proteins (NoMPs)
7.5 Links between conventional chaperones and NoMPs, possible roles for chaperones in the nucleolus
7.6 Open questions and future directions
7.7 List of abbreviations
7.8 References
Part III Nucleolar protein translocation
Chapter 8 Nucleolar localization/retention signals
8.1 Introduction
8.2 Current research
8.3 Perspectives
8.4 Acknowledgments
8.5 References
Chapter 9 Nucleolar transport of putative GTPase GNL1 and related proteins
9.1 GTPases
9.2 The YawG/YlqF family
9.3 The HSR1-MMR1 family
9.4 Subcellular localization and targeting of GNLs
9.5 Conclusion
9.6 References
Chapter 10 Nucleolar protein anchoring and translocation
10.1 Introduction
10.2 Protein anchoring
10.3 Nucleolar proteins translocation
10.4 Conclusions and perspectives
10.5 Abbreviations
10.6 References
Part IV Nucleolar proteins and disease
Chapter 11 The nucleolus as a stress response organelle
11.1 Introduction
11.2 The nucleolus and proteotoxic stress
11.3 The nucleolus, nucleolar stress and cancer
11.4 Models for p53 activation following nucleolar stress
11.5 Independent nucleolar stress pathways
11.6 Concluding remarks
11.7 Acknowledgements
11.8 Abbreviations
11.9 References
Chapter 12 The nucleolar aspect of breast cancer
12.1 Introduction
12.2 Association of nucleolus hypertrophy with breast cancer
12.3 Nucleols-associated tumour suppression
12.4 Oncogene-mediated increases in rRNA synthesis
12.5 Concluding remarks and future perspectives
12.6 References
Chapter 13 Cysteine proteinase inhibitors in the nucleus and nucleolus in activated macrophages
13.1 Introduction
13.2 Macrophages
13.3 Cystein proteinases in activated macrophages and their endogenous inhibitors
13.4 Conclusions and future directions
13.5 Acknowledgements
13.6 References
Chapter 14 Nucleolar proteins and cancer: The roles of Aurora-A-interacting nucleolar proteins in mitosis and cancer
14.1 Introduction Nucleolar proteins and mitosis
14.2 NuSAP
14.3 Eg5
14.4 Arpc1b
14.5 Aurora A as a target for cancer therapy
14.6 Perspectives
14.7 Acknowledgements
14.8 References
Chapter 15 Nucleolar transplantation and human embryogenesis
15.1 Introduction
15.2 Manipulating the oocyte nucleolus (NPB)
15.3 Nucleoli (NPBs) in oocytes and zygotes and embryonic development
15.4 Why is the nucleolus important?
15.5 Can we rescue human oocytes or zygotes without or with abnormal nucleoli?
15.6 Conclusions
15.7 Acknowledgements
15.8 References
Part V Conclusions
Chapter 16 The nucleolus: From its formation to the future
16.1 Introduction
16.2 How does the nucleolus form?
16.3 Pathophysiology, cancer and the nucleolus
16.4 Nucleolar protein targeting
16.5 Nucleolar protein translocation
16.6 The value of model systems
16.7 New approaches to research
