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About this book
PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications. This book is intended for researchers at all levels in labs involved in molecular human genetics, mammalian genetics (mouse, rat, etc), molecular microbiologists, molecular parasitologists, biochemists who use molecular biology techniques, and some molecular diagnostic labs.
1. Theories of gel electrophoresis of high molecular weight DNA; 2. PFGE in physical mapping; 3. Mutation detection and diagnosis using PFGE; 4. Cloning from PFGE-purified genomic DNA fragments and YACs; 5. Yeast artificial chromosome cloning using PFGE; 6. PFGE analysis of yeast artificial chromosomes; 7. Functional analysis of mammalian genomes using yeast artificial chromosomes; 8. PFGE in the study of a bacterial pathogen (Haemophilus influenzae); 9. Analysis of the genomes of protozoan parasites using PFGE