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RNA Viruses

Handbook / Manual

Series: Practical Approach Series

Edited By: Alan Cann

288 pages, Illus

Oxford University Press

Paperback | May 2000 | #107214 | ISBN: 0199637164
Availability: Usually dispatched within 2-3 weeks Details
NHBS Price: £79.99 $105/€91 approx
Hardback | May 2000 | #107213 | ISBN: 0199637172
Out of Print Details

About this book

RNA Viruses: A Practical Approach is wide ranging in scope, from emerging technology such as reverse genetics and retrovirus vectors, to money saving tips - how to make your own silica particles for high efficiency RNA extraction and liposomes for cell transfection! Chapter one covers the fundamentals of investigating RNA virus genome structure at a molecular level. Chapters two and three describe techniques for mutagenesis of RNA genomes and analysis of transcription. Chapter four deals with RNA virus-encoded proteinases, an important aspect of the control of RNA virus gene expression. Chapter five considers retrovirus oncogenesis and chapter six analysis of RNA virus quasispecies. Chapter seven describes systems for investigation of in vitro replication of positive-stranded viruses and chapter eight the packaging of RNA virus genomes. In addition to the technical aspects of reverse genetics and retrovirus vectors, both of the final two chapters also consider ethical aspects of these new technologies.


Investigation of RNA Virus Genome Structure; Mutagenesis of RNA Virus Genomes; Analysis of Transcriptional Control in RNA Virus Infections; Analysis of RNA Virus-encoded Proteinases; Detection and Analysis of Host Gene Targets for Oncogenic Retroviruses; Analysis of RNA Virus Quasispecies; In Vitro Replication of RNA Viruses; Packaging of Segmented and non-segmented RNA Virus Genomes; RNA Virus Reverse Genetics; Development of RNA Virus Vectors for Gene Delivery

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