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About this book
About this book
Reviews every aspect of SAGE technology (Serial Analysis of Gene Expression), including the evaluation of SAGE, the improvement and further development of SAGE methodology, the statistical analysis and mathematical modelling of SAGE data, and the practical application of SAGE in eukaryotic organisms, stem cell research and brain cells analysis.
1. Evaluation of SAGE Tags for Transcriptome Study. 2. CAGE: A Novel Approach for Rapid Gene Discovery and Gene Network Identification. 3. SuperSAGE: A Potent Transcriptome Tool for Eukaryotic Organisms. 4. An Improved Protocol for SAGE Tag-to-gene Allocation. 5. Using HPLC to Purify Ditags for SAGE Library Construction. 6. Web Tools for Statistical Analysis of SAGE Data. 7. Convert MPSS Orphan Tags into 3' ESTs. 8. Mathematical Analysis and Modeling of SAGE Transcriptome. 9. Statistical Analysis of SAGE Data. 10. Studies of Plant Gene Expression Using SAGE. 11. SAGE Analysis of Embryonic Stem Cells. 12. Determining Transcriptome Differences Between Brain Territories. 13. Identifying Selection Signatures in Mammalian Genes Through the Analysis of Patterns of Gene Expression. 14. SAGE Identifies Transcripts Involved in Enhanced Performance of Endurance Athlete's Muscle. 15. Using SAGE to Analyze Signaling and Development in Drosophila. 16. Use of SAGE Technology to Reveal Changes in Gene Expression in Arabidopsis Undergoing Cold Stress. 17. SAGE Analysis of Age- and Sex-Associated Changes in Cardiac Gene Expression. 18. Reverse Transcriptome: Identification of Novel Transcripts Through Novel SAGE Tags.