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Presents shortened versions of the methods published in Current Protocols in Molecular Biology. It includes new chapters on chromatin assembly and analysis, nucleic acid arrays, generation and use of combinatorial libraries, discovery and analysis of differentially expressed genes in single cells and cell population.
Escherichia coli Plasmids, and Bacteriophages Preparation and Analysis of DNA. Enzymatic Manipulation of DNA and RNA. Preparation and Analysis of RNA. Recombinant DNA Libraries. Screening of Recombinant DNA Libraries. DNA Sequencing. Mutagenesis of Cloned DNA. Introduction of DNA into Mammalian Cells. Analysis of Proteins. Immunology. DNA-Protein Interactions. Saccharomyces Cerevisiae. In situ Hybridization and Immunohistochemistry. The Polymerase Chain Reaction. Protein Expression. Analysis of Protein Phosphorylation. Appendices. Index.