In Single Molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action.
The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.
Chapter 1: Nanomechanics of proteins, both folded and disordered Ruben Hervas, Albert Galera-Prat, Angel Gomez-Sicilia, Fernando Losada-Urzaiz, Maria del Carmen Fernandez, Debora Fernandez-Bravo, Elena Santana, Clara Barrio-Garcia, Carolina Melero & Mariano Carrion-Vazquez
Chapter 2: Force Spectroscopy and Recognition Imaging of Cells from the Immune System Bianca L Bozna Memed Duman Peter Hinterdorfer
Chapter 3: Catch bonds of integrin/ligand interactions Cheng Zhu Wei Chen
Chapter 4: AFM visualization of protein-DNA interactions Yuri L Lyubchenko
Chapter 5: Nano-visualization of proteins in action using high-speed AFM Takayuki Uchihashi Noriyuki Kodera Toshio Ando
Chapter 6: Enzyme catalysis at the single-molecule level Raul Perez Jimenez Jorge Alegre-Cebollada
Chapter 7: Single-molecule studies of amyloidogenic proteins Miklos S.Z. Kellermayer Arpad Karsai Unige Murvai Szilvia Erdelyi-Botor Jozsef Kardos Ricardo H. Pires
Chapter 8: Stretching and Imaging Individual Proteins on Live Cells using Atomic Force Microscopy David Alsteens Yves Dufrene
Chapter 9: Individual proteins under mechanical stress: Lessons from theory and computer simulations Dmitrii E Makarov