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The use of stable isotopes in nutritional studies is now widespread, and the technique is becoming increasingly popular. Practical applications are numerous and include: calcium and iron absorption studies; studies looking at the impacts of diet, physical activity, aging, and medical therapy and supplementation on nutrient metabolism; measurement of energy cost of pregnancy; causes of growth faltering in infants and investigations into childhood and adult obesity. This book is designed as a laboratory handbook of methods used to perform stable isotope studies in humans. It covers basic principles, dosage information, sample preparation procedures, analytical instrumentation and necessary mathematical methods. It will provide the fundamentals to enable researchers to evaluate and establish stable isotope methods in their own laboratories.
The measurement of protein kinetics with stable isotope tracers; stable isotope studies of macromineral metabolism - calcium, magnesium and iron; evaluation of trace mineral status and bioavailability using stable isotopes (zinc, copper, selenium, molybdenum); energy utilization with doubly labelled water (2H218O) ; body composition measurements with 2H218O isotope dilution; stable isotopes and gas chromatoraphy - mass spectometry in studies of glucose and metabolism in children; cholesterol and other lipid metabolism. (Part contents)