This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.
PART I; * Introduction; * Seed transmission of viruses; * Ecology, epidemiology and control; PART II; * Biological assays; * Serological testing methods; * Nucleic acid-based testing methods; * Nucleic acid hybridization; * Epilogue; Appendix 1. List of seed-transmitted viruses and viroids; Appendix 2. Reagents, solutions and buffers; Appendix 3. Suppliers of laboratory equipment and materials.
S E Albrechtsen, formerly of the Danish Government Institute of Seed Pathology for Developing Countries, Copenhagen, Denmark